Cytoskeletal protein antibody development has been a precious tool in the study of cytoskeletal dynamics and their places in cell biology and disease. The process generally involves immunizing animals, such as mice or rabbits, with purified cytoskeletal proteins to generate specific antibodies. These antibodies can be used in various techniques, such as Western blotting, immunofluorescence, and immunoprecipitation, to detect and study the localization and function of cytoskeletal proteins in cells. So far, some of the achievements made in cytoskeletal protein antibody development include the identification of specific cytoskeletal proteins, improved specificity and sensitivity of antibodies, functional studies, and diagnostic and therapeutic operations. After nonstop testing and optimization, cytoskeletal proteins have been employed in diagnostic tests for various diseases and probable therapeutic interventions for diseases such as cancer and autoimmune diseases. Antibodies have been used in a variety of functional studies to understand the roles of cytoskeletal proteins in cell structure.